Your Challenges Require Smart Solutions:
In vitro & in vivo Toxicology
Performed in-house in a quality controlled way
The Pharmacelsus service portfolio on in vitro and in vivo toxicology service offers you a complete workflow. De-risk either your early stage drug discovery or late stage development programs with us. In vitro toxicology assays are performed in-house in a quality controlled way. In vivo toxicology studies according to national and international guidelines are available on rodent and non-rodent species in cooperation with our trusted partner. In vivo non-GLP rodent studies are performed in house.
In vitro Toxicology
Cytotoxicity
- Test systems
- Established cell lines and primary cells, 2D–monolayer, suspension and 3D-organoid cultures
- Analysis
- Fluorescence, luminescence, absorbance
- Indicator
- Resazurin, XTT, MTT, ATP, LDH
- Readout
- Viability, Cell Membrane Damage, energy content
Mitochondrial Toxicity
- Test Systems
- HepG2, HepG2-Oxphos
- Analysis
- Fluorescence, luminescence
- Indicator
- Resazurin, XTT, ATP
- Readout
- Viability
- Format
- 96-Well Plate/Plate Reader
- Multiplexing of analytical endpoints available
Metabolite Toxicity
- Test systems
- HepG2 w/ and w/o metabolic activation - by S9-Fraction
- Analysis
- Luminescence
- Indicator
- ATP
- Format
- 96-Well Plate/Plate Reader
- Metabolite identification via LC-MS
- Reactive metabolite trapping in liver microsomes
Immunotoxicity
- Test Systems
- PMBC, monocytes, blood
- Analysis
- Luminex technology
- Readout
- Lymphocyte subsets, cytokine levels
- Format
- 96-Well Plate/Plate Reader, Luminex
- Can be combined with other systems e.g. viability assays
- Recommended assays according to ICH S8
Hepatotoxicity
- Test systems
- HepG2, primary hepatocytes
- Analysis
- Fluorescence, luminescence, Luminex technology
- Indicator
- Resazurin, ATP
- Readout
- Viability, energy content, biomarker levels
- Format
- 96-Well Plate/Plate Reader, Luminex
Allergic Response / Histamine Release
- Test systems
- Peritoneal mastocytes
- Analysis
- Absorption
- Indicator
- O-phtaldialdehyde
- Readout
- Histamine release
- Format
- 96-Well Plate/Plate Reader
In vivo Toxicology*
Species
- Rodents
- Non-rodent species
- (please contact us)
Routes of Administration
- Oral
- Intraperitoneal
- Intravenous
- Subcutanous
- Dermal
- Rectal
- Others
- (please contact us)
Acute Toxicity
- Systemic toxicity
- Phototoxicity
- Local tolerance
- Skin sensitisation and irritation
- Others
- (please contact us)
Subchronic & Chronic Toxicity
- Rodents and non-rodents
- DRF studies
- MTD studies
- Toxicokinetics
Reproductive Toxicity
- Fertility and reproduction
Pathology
- Histology
- Immuno-histopathology
- Others
- (please contact us)
Further toxicological assays are available on request.
Please contact us for your specific toxicology study.
For In Vitro Toxicology
Dr. Julia Susewind
Project Manager ADMET & in vitro Pharmacology
susewind@pharmacelsus.de
For In Vivo Toxicology
Anika Christoffel
Team Manager in vivo Pharmacology
christoffel@pharmacelsus.de